western blotting การใช้

• Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
• Representative data of the histone modifications at the specific sites were assessed using Western blotting.
• Other techniques used in the analysis of lipid rafts include ELISA, western blotting, and FACS.
• Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
• Cell was driven to lysis and subsequent western blotting to analyse the isolated cross-linked complexes.
• Examination of a series of tonsils with Western blotting gave a 100 % detection rate for HHV-6.
• It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.
• Western blotting is a routine molecular biology method that can be used to semi-quantitatively compare protein levels between extracts.
• The detection of horseradish peroxidase by enzymatic chemiluminescence ( ECL ) is a common method of detecting antibodies in western blotting.
• Far-western blotting employs non-antibody proteins to probe the protein ( s ) of interest on the blot.
• Western blotting allows the detection of specific proteins from extracts made from cells or tissues, before or after any chemiluminescent reaction.
• These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
• Brain-region-specific calcium-dependent protein kinase C expression was assessed in control and irradiated rats using standard Western blotting procedures.
• Post-translational modification of proteins can be experimentally detected by a variety of techniques, including mass spectrometry, Eastern blotting, and Western blotting.
• Standard and conservative biochemichal methods, such as cell fractionation and western blotting would not have revealed the partition into the two types of cytosolic aggregates.
• These cells were grown in a high-glucose medium, from which a sample of 3 cm2 was removed to proceed with the western blotting.
• Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting.
• A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.
• Crosslinked protein complexes can be detected by decreased mobility on SDS-PAGE followed by Western blotting, size exclusion chromatography, sucrose density gradient sedimentation or mass spectrometry.
• Reagents are created for use in flow cytometry, ELISA, immunoprecipitation, Western blotting, immunofluorescence microscopy, immunohistochemistry, and in vitro or in vivo functional assays.